3. Ordering information. 4. Functional diagram. HEFB. Quad 2-input AND gate. Rev. 8 — 15 December Product data sheet. Table 1. Ordering. Details, datasheet, quote on part number: ZL Application Notes) Ordering Information ZL/DCE ZL/DCF (tubes) 8 pin SOIC (tape and reel) 8. Cat. No. / Cat. No. / System (w/micro CA). Cat. No. Cat. No. / Cat. No. / Removable Drum Only.
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Alexa Fluor is a registered trademark of Life Technologies Corporation. Would you like to visit your country specific website? Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser of 4018 STING protein. Incubate for at least 5 min at room temperature. Datasheft optimal lysate concentration will depend on the expression level of the protein of interest.
Application Dilutions Immunofluorescence Immunocytochemistry 1: Rinse three times in 1X PBS for 5 min each. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Blotting Membrane and Paper: Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
Solutions and Reagents Achieve higher quality immunofluorescent images dataeheet the efficient and cost-effective, pre-made reagents in our Immunofluorescence Application Solutions Kit NOTE: ATP 10 mM for kinase assays: From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Repeat washing step once more.
For best results, allow mountant to cure 440818 at room temperature.
Do not aliquot the antibody. Antibodies are purified by protein A and peptide affinity chromatography. More about how we get our images. To Purchase S View sizes. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.
Wash three times for 5 min each with 15 ml of TBST. Pre-clear enough lysate for test samples and isotype controls.
Ubiquitinating enzymes UBEs catalyze protein ubiquitination, datashest reversible process countered by deubiquitinating enzyme DUB action 1,2. Scrape cells off the plate and eatasheet to microcentrifuge tubes. Remove PBS and add 0. Primary Antibody Dilution Buffer: Incubate with rotation for 20 min at room temperature. Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1—2 hr at room temperature in the dark.
Analyze cells in DNA staining solution on flow cytometer.
CST – Phospho-STING (Ser) (D8K6H) Rabbit mAb
Wash cells by centrifugation in excess 1X PBS to remove methanol. Recommended Anti-Rabbit secondary antibodies: Would you like to visit your country specific website?
Do not aliquot the antibody. Aliquot desired number of cells into tubes or wells. Incubate 30 min on ice.
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While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.
More about how we get our images.