Download Citation on ResearchGate | On Jul 31, , Suzanne M. Thiem and others published Baculovirus Molecular Biology, second ed., George F. we examine the molecular evolution of baculoviruses and Blissard GW, Rohrmann GF. Baculovirus diversity and molecular biology. Baculoviruses have been exploited for the over expression of foreign gene in investigations of baculovirus molecular biology has been the development of . Vanarsdall, A.L., Pearson, M.N. and Rohrmann, G.F. () Characterization of.
|Published (Last):||25 January 2006|
|PDF File Size:||6.86 Mb|
|ePub File Size:||4.10 Mb|
|Price:||Free* [*Free Regsitration Required]|
In this investigation, the homologous region in the Env protein of the gypsy retrovirus of Drosophila melanogaster DmegypV was investigated. This has led to the investigations of two previously uncharacterized viruses; one of alpacas and the other pathogenic for rabbits. Laura Esteban for their helping with the paper.
To better characterize the role of these genes in the baculovirus replication cycle, quantitative DNA replication assays were performed and demonstrated that in cells transfected with the Ac, or knockouts, DNA replicated with mopecular kinetics as a control virus.
Phylogenetic analyses based on core gene sequences and the gene composition of the genomes supported the current division of the Baculoviridae into 4 genera: The data suggest that the AN endonuclease may digest the intermediates in replication and recombination at positions of structural irregularities in DNA duplexes, whereas LEF-3 may further regulate processing of the intermediates by AN rohrmann the endonuclease and exonuclease pathways.
We are also collaborating with scientists in the College of Veterinary Medicine to investigate virus infections of livestock. This information may lead to an understanding of whether these viruses are present in vaccine preparations and, if they are present, whether they can transpose into or infect human and other types of cells. Our current evidence suggests that alkaline nuclease is involved in recombination-dependent replication and is required for the production of full length genomes.
In gel retardation assays, DBP showed a high affinity for single-stranded oligonucleotides and was able to compete with another baculovirus SSB protein, LEF-3, for binding sites.
Baculovirus Molecular Biology [Internet].
These estimations were inferred rohrmanh Blast P algorithm http: Turn recording back on. The resulting order of core genes shows that pif-2 was the most conserved baculovirus ancestral sequence, whereas desmoplakin was the gene with evidence of greatest variability.
Registration Forgot your password? This proved that the endonuclease rohrjann an intrinsic activity of baculovirus AN. The Baculoviridae family is divided into four genera according to common biological and structural characteristics: They also show promise as viral insecticides and as vectors for gene therapy. To investigate the role of the gene products encoded from the open reading frames, and of Autographa californica multiple nucleopolyhedrovirus AcMNPVa set of bacmid knockout and repair constructs were generated.
Baculovirus core gene variability. We have also used mass spectrometry to determine the size and sequence of fragments of proteins derived from tryptic digests. However, other sequences like pif-2 gene would not accumulate mutations because the protein encoded might lose vital functions not necessarily associated with the nature of the host. These include characterization of the 21 amino acid predicted fusion peptide sequence that is highly homologous to a region of baculovirus envelope fusion proteins.
The studies on the envelope fusion protein provide information on how retroviruses likely evolved from non-infectious transposable elements to infectious viruses.
Bioinformatics Needs for the post-genomic era Dr. LEF-3 is a multifunctional protein that appears to play a pivotal role in a variety of processes related to baculovirus DNA replication. This activity likely generates single stranded ends of DNA fragments that then initiate recombination by strand invasion.
In this project we will map the structural and functional domains of LEF To characterize DBP, we expressed it as an N-terminal His6-tag fusion using a recombinant baculovirus and purified it to near homogeneity.
We have characterized a protein called very late expression factor -1 vlf-1 and demonstrated that it is capable of binding to cruciform structures suggesting that it may be involved in the resolution of structures generated during DNA replication. A Baculovirus alkaline nuclease knock out construct produces fragmented DNA and aberrant capsids. And the same can be observed when analyzing Group I, where the presence of two different clades can be clearly inferred clade a and clade b.
Western blot analyses of budded virus from cells infected with the repair viruses showed that these proteins are associated with the viral nucleocapsid. We were able to accomplish this by the application of bacterial artificial chromosome BAC technology to the study of baculoviruses. Transcriptional mapping of two genes encoding baculovirus envelope-associated proteins.
We have found about 12 lineages of these viruses in Spodoptera frugiperda cells and also have investigated their presence in Trichoplusia ni cells.
International Journal of Evolutionary Biology
The research on veterinary pathogens is important in preventing the losses caused by these viruses in farm animals. The analysis shows that Group I alphabaculoviruses and gammabaculoviruses have a lower diversity of gene content with respect to the rest of lineages. Mostly, the evolutionary inferences were in agreement with much stronger subsequent studies based on sequence analyses derived from sets of genes with homologous sequences in all baculoviruses.
Then, the fact that a viral family consists of members who share a common pattern of genes and functions and whose proliferation cycle continuously challenges the viral viability turns it essential to take into account their higher or lesser tolerance to the molecular changes. The predicted Env protein of insect retroviruses errantiviruses is related to the envelope fusion protein of a major division of the Baculoviridae. The number and identity of shared orthologous genes in every accepted member of each genus were investigated, and the unique sequences typical of each clade as well as those shared between different phylogenetic groups were identified Figure 2.
This figure is based on the data of. However, we have not been able to identify any enzymatic activity.
Baculovirus Molecular Biology