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BIOASSAY OF RABIES VACCINE PDF

It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. Three rabies vaccines for human use derived from different rabies virus strains Animals; Biological Assay/methods*; Biological Assay/standards; Biological. BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.

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Upload from Desktop Single File Upload. Ratio between two dose of test and two dose of standard should be equal This ratio kept constant through out the assay. The potency of rabies antiserum is determined by comparing a lethal intracerebral rabied of rabies virus with the dose of standard preparation of rabies antiserum necessary to give same protection.

The prepared plates or dishes must be stored such that no significant growth of the test microorganism occurs before use, and the surface of the agar layer is dry at the time of use. Incubation time days. Dephtheria anti-toxin is preparation containing antitoxic globulins that they have the power of specifically neutralizing the toxin formed by Cornebacterium diphtheria.

Vaccines |authorSTREAM

Standard preparation is dried serum or Other preparationvacclne potency of which has been determined in relation to international standard.

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The toxin should be selected for assay. Prepare fold dilution of std. Potency of rabies vaccine is determined by comparing the dose necessary to protect mice against a lethal intracerebral dose of rabies vaccine necessary to provide the same protection.

Stay min protected from the light.

The test toxin is allowed to stand for some months before being used for the assay of samples bioassag antitoxin. Go to Application Have a question? Automatically changes to Flash or non-Flash embed. These two groups are used for titration of LD50 of challenge suspension. Suggested inoculums composition amount – 0.

Rabies vaccine is a suspension of a suitable strain of fixed rabies grown in suitable approved cell culture and inactivated by a suitable method. Three rabies vaccines for human use derived from different rabies virus strains and prepared on different cell culture substrates were compared with the International Standard for Rabies Vaccine using in vivo and in vitro assay methods in bioassaj collaborative study involving 14 participants.

Measure all the response and calculate the result of the vacfine by standard statistical method. Prepare standard preparation with saline solution final concentration Dose should be added at regular interval[minutes].

PHARMACOLOGY: Biological Assay of Diphtheria Antitoxin

Inject the Virus 0. On the other hand another vaccine, derived from the LEP strain, was considerably more potent in antigenicity assays than in immunogenicity assays. Dilute 25ml of basal medium stock solution with equal volume of H2O sterile in autoclave. Never has been confirmed Rabies virus never isolated from blood. Oxytocin stimulate the contraction of the uterine smooth muscle and the memory gland.

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Any suitable strain rabies virus of known potency, such as the CVS strain may be used. Acid digested casein solution. P Exposure the gluteus primus muscle thigh and remove politeal artery and crural vein.

Protected from the light. Then they are incubated or about 24 hours at about and the diameter or areas of the circular inhibition zones are measured.

Selection of test toxin: LP means limes paralyticum This is the smallest quantity of the toxin when mixed with 0. Presentation O No description available.

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The presentation is successfully added In Your Favorites. Prepare diphtheria toxin by filtering through bacteria-proof filter the medium in which a toxigenic strain of C.

Any suitable strain rabies virus of known potency, such as the CVS strain may be used. Bite from an infected animal. The presentation is successfully added In Your Favorites.

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